Coding

Part:BBa_K5301007:Design

Designed by: Xiaowen Sun   Group: iGEM24_BNU-China   (2024-09-22)


SnTST-mCh[11] is one of the components of multi-polymerized MSP.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 757
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 276
    Illegal BglII site found at 648
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To avoid self-cyclization of MSP fragments caused by the cross-reaction between SdyCatcher and mCherry[11], we placed SnoopTag and SpyTag at the N-terminal and C-terminal of the third components of multi-polymerized MSP.

Source

SnoopTag is derived from RrgA, an adhesin from Streptococcus pneumoniae. SpyTag is derived from Streptococcus pyogenes fibronectin-binding protein, FbaB. mCherry [11] is the C-fragment of mCherry fluorescent protein. NW15 is the truncated form of NW30, which is originated from a set of α-helixes derived from apolipoprotein A-1 (ApoA1).

References